Peptide synthesis workflow is a multi-step process which involves synthesis, purification, and evaporation to obtain the desired peptide product. This demands that the steps from amino acid building blocks to final purified peptide product proceed as efficiently and smoothly as possible. In the last couple of years automated SPPS was optimized in various ways, enabling peptides to be obtained faster, in higher throughputs or larger amounts.
Scientists want to get their peptides always faster, but a shorter synthesis time alone is insufficient and not the most optimized way to accomplish this.
Purification and evaporation issues can dramatically impact the efficiency of the workflow and are the cause of many bottlenecks often disregarded by scientists.
Preparative reversed-phase HPLC has been so far the method of choice for purification of synthetic peptides, but is limited by small loading amounts, long separation times, poor recoveries, and high costs.
Similarly, freeze drying of peptides, despite being a crucial step for the whole process, is generally accepted as time-consuming and requires special equipment as well as a reliable access to liquid nitrogen supply.
This presentation will feature strategies to improve the peptide synthesis workflow, which enable the synthesis of highly pure crude peptides, demonstrate alternative techniques to rapidly purify crude peptide mixtures and highlight methods to quickly concentrate solutions along the entire peptide synthesis workflow.
We will show that these improvements combined together can lead to a substantial decrease in the time needed to obtain pure peptides, hence accelerating the whole research cycle.